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IMMunOHIstOcHMEIcal ExPrEssIOn Of clauDIn 1& 3 In RADICULAR CyST, ODONTOGENIC KERATOCyST, UNICySTIC AMELOBLASTOMA AND CONVENTIONAL AMELOBLASTOMA

Hend Mohammed wagieh, Safa Fathy Abd El-Ghani and Asmaa Emad El-Din Mohammed

ABSTRACT

Odontogenic cysts and tumors have a wide spectrum of histopathologic and clinical characteristics, ranging from benign non-invasive to locally invasive or malignant lesions. However, little is elucidated about their pathogenesis.

The aim of study was to evaluate the immunohistochemical expression of claudin-1 and claudin-3 in the most common odontogenic lesions. Comparing the expression levels of these two markers among those lesions and finally, correlation of the obtained results with their biological behaviors were also performed.

Forty  archival  cases  of  radicular  cyst,  odontogenic  keratocyst,  unicystic  ameloblastoma and ameloblastoma (10 cases each) were stained with hematoxylin and eosin for histological examination. Streptavidin-biotin method was used for immunohistochemical staining by anti claudin-1 and anti claudin-3 antibodies.

Our results revealed that the expression level of both markers was gradually decreased starting from radicular cyst to odontogenic keratocyst, unicystic ameloblastoma until reaching its lowest value in ameloblastoma. the statistical analysis of the current work revealed a highly significant difference in area percentage of both markers among the examined groups. In addition, the area percentage of claudin-1immunoexpression was much higher than that of claudin-3, and the correlation between both markers was positive.

In conclusion, Claudins-1 and -3 could be useful prognostic biomarkers in odontogenic lesions. however, claudin-1 is more specific for odontogenic lesions than claudin-3. Besides, they could aid in differentiation of the neoplastic from non-neoplastic odontogenic lesions and could be auxiliary indicators of aggressiveness and invasion potentials.

KEy wORDS: Radicular cyst, Odontogenic keratocyst, Unicystic Ameloblastoma, Ameloblastoma.

Vol. 64, 281:287, January, 2018

I.S.S.N  0070-9484

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EVALUATION OF VERTICAL RIDGE AUGMENTATION USING: PRE-SHAPED COLLAGEN MEMBRANE (ZIMMER CURV) VS LOw PROFILE TITANIUM MESH IN ATROPHIC POSTERIOR MANDIBLE A RANDOMIZED CLINICAL TRIAL

Maha Sayed Mostafa Darwish, Mohamed Galal Bihery , Mostafa Ibrahim Shendy and Ahmed Mohamed Salah

ABSTRACT

Aim: Evaluation of the efficacy of the pre-shaped resorbable membrane on increasing height of atrophic posterior alveolar ridge in mandible vs low profile titanium mesh by using cBct scan.

Patients and Methods: The study was conducted on 8 bilateral posterior mandibular sides of eight patients which were suffering from severe mandibular atrophy. Each patient was treated by vertical ridge augmentation by autogenous cortico-cancellous bone graft taken from anterior iliac crest of the same patient. In one mandibular side of each patient bone graft protected with titanium mesh (control group) and the other side in the same patient protected with pre-curved membrane (study group).The patients were followed up for six months postoperatively clinically and by cone beam computed tomograms immediately postoperative, which represented the base line radiographs and after six months, bone height was measured to compare amount of bone resorption of both groups.

Results: all statistical tests were two sided and performed at a significance level of ^5; =.05 and by using Student’s T-test (Student’s t-test: t = 2.414, p < .05). The results showed a statistically significant difference between the resorption rate rr of the vertically augmented alveolar ridge under the membrane in the study group (n=8) and RR in the control group (n=8). The results showed increase in resorption rate in the study group more than the control group.

Conclusion: The resorption rate in augmented bone is higher in sides where the membrane was used than the sides in which the Ti- mesh was used because the membrane loses its form and its rigidity by humidity before consolidation of the augmented bone.

KEy wORDS:  Pre-shaped Collagen membrane, Vertical ridge augmentation, Atrophic mandible, Guided bone Regeneration, titanium mesh.

Vol. 64, 273:279, January, 2018

I.S.S.N  0070-9484

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ACCURACy OF PLANMECA PROMAX 3D MID® CONE BEAM COMPUTED TOMOGRAPHy MACHINE IN MAXILLARy LINEAR MEASUREMENTS; DIAGNOSTIC ACCURACy STUDy

Amr Taha, Ahmed M. Abd Alsamad, Hany Mahmoud Omar and Mushira M. Dahaba

ABSTRACT

Objectives: assessment of the accuracy of linear measurements in maxilla obtained by CBCT scanner (Planmeca Promax 3D MID CBCT machine and software) in comparison with the real measurement obtained from the dry skulls

Methods: – The study was performed on thirty (30) dry skulls obtained from the Anatomy Department of Faculty of medicine, Cairo University. The skulls attached to their mandibles were radiographed using Planmeca ProMax 3D Mid CBCT machine. Real measurements were performed using a digital caliper. The results obtained was subjected to Intra- and inter-observer agreement was assessed using intra-class correlation coefficient (icc).Bland-altman plot (differences-vs- means plot) used to show the agreement between two quantitative measurements by studying the mean difference and constructing limits of agreement .

Results: excellent correlation was shown between the maxillary CBCT linear measurements and real measurement obtained from dry skulls. There was excellent inter and inter observer reliability.

Conclusion:-Maxillary linear measurements driven from CBCT images of Planameca Promax 3D Mid® machine are accurate, reproducible and reliable for all maxillofacial applications.

KEy wORDS: Cone beam computed Tomography, CBCT, Linear measurement, Planmeca ProMax 3D

Vol. 64, 267:272, January, 2018

I.S.S.N  0070-9484

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COMPARISON BETwEEN IMMEDIATE AND DELAyED IMPLANT PLACEMENT FOLLOwING SPLITTING AND GRAFTING By (HA) FOR ATROPHIC ANTERIOR MAXILLA

Nour Eldeen Mohamed, Layla Mostafa Omara, Mohamed El-Faramawey and Hesham Elsayed Elhawary

ABSTRACT

Aim of the study: The purpose of this study was to compare the survival rate of immediate & delayed placement of dental implants, following narrow maxillary ridge splitting & grafting by an alloplastic material.

Materials and methods: The present study was conducted on 6 patients using 16 implants. The patients were divided randomly into 2 groups, Group I patients: eight sites were subjected to maxillary ridge splitting(anterior region), ridge grafting with Hydroxyapatite and delayed placement of total number of eight implants while group II patients: Eight sites were subjected to maxillary ridge splitting, ridge grafting with Hydroxyapatite followed by immediate placement of total number of eight implants. Preoperative, immediate (one week), 3 months and 6 months post-expansion and insertion CBCT radiographs assessed for measuring the bucco-lingually width.

Results: During the follow up period, all patients showed no post-operative complications, wound healing was uneventful in all patients, without any signs of wound dehesince or infection, All implants inserted were stable, which was detected by the periotest. The CBCT radiograph showed proper contact between bone and implant, also showed increase buccolingual width for both groups immediately post-operative and decrease in the width 6 months post-expansion and insertion of implants.

Conclusion: The recent work has shown that ridge splitting technique can be a useful method for managing the narrow maxillary residual ridge in both groups.

Vol. 64, 259:265, January, 2018

I.S.S.N  0070-9484

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EVALUATION OF MICROSHEAR BOND STRENGTH OF SELF ADHERING FLOwABLE COMPOSITE AND CONVENTIONAL FLOwABLE COMPOSITE ON DENTINE OF PRIMARy TEETH: IN VITRO STUDy

yasmine Ashraf El Banna, Sherif Bahgat El Taweel and Sara Ahmed Mahmoud

ABSTRACT

Aim: testing the microshear bond strength of self adhering flowable composite and conventional flowable composite to dentine of primary teeth.

Materials & methods: Thirty sound human deciduous canines were collected, the collected teeth were indicated for extraction due to normal shedding, over retention and orthodontic reasons. Teeth were assigned into three groups according to the restorative materials used (n=10), Group a:  self adhering flowable composite (fusio™liquid  dentin),  Group B: self adhering flowable composite (Fusio™Liquid Dentin) + self etch adhesive (Single Bond Universal), Group C: conventional flowable composite (filtek™ Z350 Xt flowable) + self etch adhesive (Single Bond Universal). Teeth were restored for microshear bond strength testing then samples were stored in isotonic saline solution for 24 hours. Then microshear bond strength was tested using universal testing machine; load was applied till specimen’s fail.

Results: Group B showed the highest bond strength values while Group A showed the lowest bond strength values (P≤0.001).

Conclusions: Self adhering composite applied according to manufacturers’ instructions produced the least bond strength values to dentine of primary teeth. However, its bonding performance improved significantly after preceding it with self-etch adhesive system.

KEywORDS: Self adhering; self etch; composite; microshear; primary teeth dentine.

Vol. 64, 253:258, January, 2018

I.S.S.N  0070-9484

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EFFECT OF LOw TEMPERATURE DEGRADATION ON THE FLEXURE STRENGTH OF SOME RECENT CERAMIC BLOCKS

Mariam Adel Hosny Mohamed, Rasha Mohamed Abdel Raouf and Nour El Din Ahmed Habib

ABSTRACT

Aim: Investigate low temperature degradation (aging ) effect of three ceramic CAD/CAM block materials: lithium disilicate(LD), translucent zirconia(TZ) and zirconia reinforced lithium silicate(Zld) on their flexure strength.

Material and methods: Fourteen disc shaped specimens were prepared from each material(12mm diameter and 1.2 mm thickness). Seven specimens per material were subjected to Low temperature degradation. while the other seven were considered as control. All specimens were subjected to biaxial flexure strength testing.

Results: tZ had the highest flexure strength while there was no difference between the others.

Flexure strength of the three materials was not affected by aging Conclusions: aging did not affect the flexure strength. translucent zirconia has the highest flexure strength while no difference between lithium disilicate and zirconia reinforced lithium silicate.

KEywORDS: Low temperature degradation, Fexure strength, CAD/CAM.

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REMINERALIZING EFFICACy OF BIOMIMETIC SELF-ASSEMBLING PEPTIDE VERSUS CASEIN PHOSPHOPEPTIDE AMORPHOUS CALCIUM PHOSPHATE AND FLUORIDE BASED DELIVERy SySTEMS IN ARTIFICIALLy INDUCED ENAMEL LESIONS: AN IN VITRO STUDy

Dina Kamal, Dina Elkassas and Heba Hamza

ABSTRACT

Objectives: investigate the efficacy of biomimetic self-assembling peptide (p11-4) on enamel remineralization compared to casein phosphopeptide amorphous calcium phosphate (CPP-ACPF) and fluoride based delivery systems.

Methods: artificially demineralized enamel windows  were created on the  buccal surfaces of forty extracted human molars.. Specimens were randomly assigned to 4 groups (n=10) according to the remineralizing agent used: g1- (control, artificial saliva), g2- (fluoride varnish), G3-(CPP-ACPF varnish), G4-(Self-assembling peptide agent).. All products were applied according to the manufacturer’s instructions and specimens were stored in a daily renewed artificial saliva. Surface microhardness (SMH) was assessed at baseline, after demineralization, after 1 week and 4 weeks remineralization.

Results: self-assembling peptide showed the highest statistically significant sMh followed by fluoride and cpp-acpf while the lowest sMh as found in artificial saliva. however, no statistically significant difference was found between fluoride and cpp-acpf. higher statistically significant SMH was found after 4 weeks compared to 1 week remineralization in all groups.

Conclusions: self-assembling peptide confers the highest remineralizing efficacy compared to fluoride and cpp-acpf; showing a promising, non-invasive regeneration potential. also, extended period of time helped to attain more benefits from the remineralizing regimens applied.

Vol. 64, 239:246, January, 2018

I.S.S.N  0070-9484

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EVALUATION OF LEVEL OF TNF-α IN CHRONIC PERIODONTITIS PATIENTS wITH GESTATIONAL DIABETES MELLITUS AFTER PHASE I PERIODONTAL THERAPy

Omneya M. Elkadi, Gihane Gharib Madkour, Hala Salem Elmenoufy and Mahmoud El Refai

ABSTRACT

Background: There is little number of studies that address the inter-relationship of gestational diabetes mellitus (GDM) and periodontitis during pregnancy. Hence, this study was conducted to provide further evidence in the possible association between chronic periodontitis and GDM by evaluating the level of tumor necrosis factor-alpha (tnf-α) of chronic periodontitis patients with GDM after phase I periodontal therapy.

Subjects and methods: This study was conducted on 40 subjects divided into 2 groups:

20 pregnant females suffering from gestational diabetes mellitus associated with moderate to severe chronic periodontitis and 20 systemically free pregnant females suffering from moderate to severe chronic periodontitis. The periodontal status of the subjects was assessed at baseline before phase I periodontal therapy and 2 months after completion of the treatment: All subjects have been screened by comprehensive periodontal examination and full periodontal charts were obtained. The following clinical parameters were assessed to determine the clinical periodontal status of patients: Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PPD), and Clinical Attachment level (cal). gingival crevicular fluid (gcf) and serum samples were collected from both study groups to detect tnf-α levels. regarding the assessment of tnf-α, real-time reverse-transcriptase Polymerase Chain Reaction (RT) PCR technique was used.

Results: results  of the present study observed statistically significant reduction in tnf-α levels after 2 months from phase I periodontal therapy. The current study showed that, there was a statistically significant positive (direct) correlation between tnf-α level, pi, gi, ppd and cal measurement at baseline and after 2 months from phase I periodontal therapy.

Conclusion: it is shown that the levels of tnf-α in gcf and serum before treatment were higher in gdM group (group 1) than in the control group. thus, the examination of tnf-α may enhance the understanding of pathogenesis of periodontitis and GDM and its assessment in the treatment process may result in better control of the disease.

KEywORDS: chronic periodontitis, gdM, tnf-α.

Vol. 64, 229:238, January, 2018

I.S.S.N  0070-9484

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ALVEOLAR CLEFT RECONSTRUCTION USING TISSUE ENGINEERED CONSTRUCTS (CLINICAL AND BONE DENSITy ASSESSMENT)

walaa Kadry, Mervat El Deftar, yasmine Nassar and Maha M Hakam

ABSTRACT

Aim of the study: To clinically and radiographically (as regards bone density) assess the outcomes of unilateral alveolar clefts grafted with tissue engineered constructs.

Patients and methods: this study was conducted on five patients presented with unilateral alveolar clefts and indicated for secondary alveolar bone grafting. The alveolar clefts were grafted with tissue engineered constructs composed of osteogenically differentiated mesenchymal stem cells (MSCs) derived from the patient’s bone marrow and loaded on collagen scaffolds (Osteovit). Postoperative clinical follow-up and a radiographic 6 months postoperative follow-up using CT scan was performed at 6 months follow up period. The newly formed bone density was measured from the CT scans and compared to the native bone density on axial CT scans.

Results: The newly formed bone density was (312.84 HU) compared to the native bone (495.39 HU).

KEywORDS: Tissue engineering, alveolar cleft, bone density.

Vol. 64, 223:228, January, 2018

I.S.S.N  0070-9484

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COLOUR CHANGE OF NANOHyBRID AND NANOFILLED COMPOSITES wITH OR wITHOUT BLEACHING AFTER IMMERSION IN COFFEE SOLUTIONS: AN IN VITRO STUDy

Randa Abdel Rahman El Naggar, M.Adel Ezzat, M. Fouad Haridy and Shereen Hafez

ABSTRACT

Objective: This study was carried out to evaluate the effect of bleaching on the colour change of two types of composite before and after immersion in coffee staining solutions.

Materials and methods: a total of 144 specimens were prepared in a circular teflon mould (10 x 2mm), then they were  divided into 2 equal groups according to composites used Amaris and Z350xT , then they were divided to three subgroups  according to the bleaching protocol ; no bleaching, bleaching after staining and bleaching before and after staining and then each group was further divided into three classes according to the staining solutions used ;artificial saliva, coffee and coffee with sugar and creamer. Colour measurements took place at baseline, after bleaching and after staining using x-rite spectrophotometer according to the CIE L*a*b* colour space.

Results: The results revealed that Amaris has the highest mean colour change, bleaching has a non-significant effect on the colour stability of composite resin but it can lighten the colour of the stained composite and finally results revealed that coffee has the highest mean colour change among the used staining solutions.

Conclusion:  nanofilled  composites  are  more  colour  stable  than  nanohybrid  composites, also bleaching is a viable option to remove extrinsic stains from resin composite.

Vol. 64, 215:222, January, 2018

I.S.S.N  0070-9484

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