Sally Kamal El-Din Mohamed, Nevine Gamil Waly, Eman H A Aboul-Ezz And Mohammed Ayad Abdel Hameed,

ABSTRACT
SHED Were Isolated From Deciduous Teeth. Cells Acquired From All Patients Were Cultured Under The Same Conditions In The Media With 2% Of FCS Cultivation Media Supplemented With ITS And Growth Factors (PDGF-BB, EGF) And Passed Three Times Per Week Over 55 Population Doublings. Dissociated Single Cells Were Used For Flowcytometric Analysis And Examined Immunohistochemistry. Cell Viability And Other Biological Properties Were Examined Periodically Every Passage Using Vi-Cell Analyzer And Z2-Counter. DNA Analysis And Phenotyping Were Done Using Flowcytometry. The Data Presented Here Demonstrate That Postnatal Dental Pulp Contains Cells That Are Clonogenic, Highly Proliferative, Properties That Effectively Define Them As Stem Cells.