Salma K. Rizk, Gihan A. Abd Elrahman, Shaymaa I. Habib And Ahmed G. Hegazi,

ABSTRACT
Objective: This Study Aimed To Evaluate The Effect Of Ethanolic Extract Of Propolis (EEP) Addition On Resin Based Dental Sealant With 10% Concentration As Regard Its Antibacterial Effect On Streptococcus Mutans In Comparison To Fluoride Releasing Sealant And Conventional (fluoride-free) Resin-based Pit And Fissure Sealant. Methodology: A Total Number Of 90 Specimens Were Prepared, Specimens Were Randomly Assigned Into 3 Groups (30 Specimens Per Group). Group I: Resin-based P&F Sealant Containing 10% EEP, Group II: Fluoride Releasing Resin-based P&F Sealant And Group III: Conventional Resin-based P&F Sealant. The Antibacterial Activity Of The Differently Investigated Groups Against Streptococcus Mutans Strain Was Evaluated Using Agar Diffusion Test. This Test Was Conducted After 1,7,14,21 And 30 Days Of Incubation In Artificial Saliva At 37�C To Mimic The Clinical Situation. Data Were Statistically Analyzed. Results Of This Study Showed Statistically Significant Difference Between The Three Investigated Groups After 1, 14, 21and 30 Days. With The Highest Mean Value For The Propolis Containing Group After 1 Day, Followed By The Fluoride Containing Group.

Conclusions: When Propolis Was Added To Resin-based Sealant, A Pronounced Antibacterial Effect Against Streptococcus Mutans Bacteria Was Evidenced.