A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_URI::$config is deprecated
Filename: core/URI.php
Line Number: 101
Backtrace:
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Router::$uri is deprecated
Filename: core/Router.php
Line Number: 127
Backtrace:
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: Cannot modify header information - headers already sent by (output started at /home1/ab8102/public_html/edjcode/system/core/Exceptions.php:271)
Filename: core/Security.php
Line Number: 274
Backtrace:
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$benchmark is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$hooks is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$config is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$log is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$utf8 is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$uri is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$exceptions is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$router is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$output is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$security is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$input is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$lang is deprecated
Filename: core/Controller.php
Line Number: 75
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$load is deprecated
Filename: core/Controller.php
Line Number: 78
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Return type of CI_Session_database_driver::open($save_path, $name) should either be compatible with SessionHandlerInterface::open(string $path, string $name): bool, or the #[\ReturnTypeWillChange] attribute should be used to temporarily suppress the notice
Filename: drivers/Session_database_driver.php
Line Number: 129
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Return type of CI_Session_database_driver::close() should either be compatible with SessionHandlerInterface::close(): bool, or the #[\ReturnTypeWillChange] attribute should be used to temporarily suppress the notice
Filename: drivers/Session_database_driver.php
Line Number: 280
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Return type of CI_Session_database_driver::read($session_id) should either be compatible with SessionHandlerInterface::read(string $id): string|false, or the #[\ReturnTypeWillChange] attribute should be used to temporarily suppress the notice
Filename: drivers/Session_database_driver.php
Line Number: 151
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Return type of CI_Session_database_driver::write($session_id, $session_data) should either be compatible with SessionHandlerInterface::write(string $id, string $data): bool, or the #[\ReturnTypeWillChange] attribute should be used to temporarily suppress the notice
Filename: drivers/Session_database_driver.php
Line Number: 208
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Return type of CI_Session_database_driver::destroy($session_id) should either be compatible with SessionHandlerInterface::destroy(string $id): bool, or the #[\ReturnTypeWillChange] attribute should be used to temporarily suppress the notice
Filename: drivers/Session_database_driver.php
Line Number: 297
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Return type of CI_Session_database_driver::gc($maxlifetime) should either be compatible with SessionHandlerInterface::gc(int $max_lifetime): int|false, or the #[\ReturnTypeWillChange] attribute should be used to temporarily suppress the notice
Filename: drivers/Session_database_driver.php
Line Number: 335
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: ini_set(): Session ini settings cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 282
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: session_set_cookie_params(): Session cookie parameters cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 289
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: ini_set(): Session ini settings cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 304
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: ini_set(): Session ini settings cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 314
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: ini_set(): Session ini settings cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 315
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: ini_set(): Session ini settings cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 316
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: ini_set(): Session ini settings cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 317
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: ini_set(): Session ini settings cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 375
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$db is deprecated
Filename: core/Loader.php
Line Number: 396
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_DB_mysqli_driver::$failover is deprecated
Filename: database/DB_driver.php
Line Number: 371
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: session_set_save_handler(): Session save handler cannot be changed after headers have already been sent
Filename: Session/Session.php
Line Number: 110
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: Warning
Message: session_start(): Session cannot be started after headers have already been sent
Filename: Session/Session.php
Line Number: 143
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$session is deprecated
Filename: core/Loader.php
Line Number: 1283
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 7
Function: __construct
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Javascript::$CI is deprecated
Filename: libraries/Javascript.php
Line Number: 81
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 9
Function: library
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Jquery::$CI is deprecated
Filename: Javascript/Jquery.php
Line Number: 110
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 9
Function: library
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: strpos(): Passing null to parameter #1 ($haystack) of type string is deprecated
Filename: libraries/Javascript.php
Line Number: 657
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 9
Function: library
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$jquery is deprecated
Filename: core/Loader.php
Line Number: 1283
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 9
Function: library
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Javascript::$js is deprecated
Filename: libraries/Javascript.php
Line Number: 86
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 9
Function: library
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$javascript is deprecated
Filename: core/Loader.php
Line Number: 1283
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 9
Function: library
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$form_validation is deprecated
Filename: core/Loader.php
Line Number: 1283
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 10
Function: library
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$mode is deprecated
Filename: core/Loader.php
Line Number: 358
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 13
Function: model
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property Edj::$Image_model is deprecated
Filename: core/Loader.php
Line Number: 358
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 14
Function: model
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$benchmark is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$hooks is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$config is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$log is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$utf8 is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$uri is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$exceptions is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$router is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$output is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$security is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$input is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$lang is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$load is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$db is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$session is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$jquery is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$javascript is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$form_validation is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$mode is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
A PHP Error was encountered
Severity: 8192
Message: Creation of dynamic property CI_Loader::$Image_model is deprecated
Filename: core/Loader.php
Line Number: 931
Backtrace:
File: /home1/ab8102/public_html/edjcode/application/controllers/Edj.php
Line: 139
Function: view
File: /home1/ab8102/public_html/edjcode/index.php
Line: 315
Function: require_once
EDJ
INFLUENCE OF QUERCETIN ON SQUAMOUS CELL CARCINOMA CELL LINE (HEP-2). AN IN-VITRO STUDY
,
Volume 2 No 4
795
Amr Helmy Moustafa El Bolok, Safaa M. Tohamy, Hanan Eid Gamal, Enas Alaa Eldin And Basma Mahmoud Ryad ,
ABSTRACT
Background: Quercetin Is An Effective Cytotoxic Anticancer Agent And Has Been Used For The Treatment Of Variety Of Malignancies. The Aim Of The Current Study Is To Investigate The Influence Of Quercetin On Squamous Cell Carcinoma Cell Line (HEP-2) After 24 Hours.
Material & Methods: Treatment Of Cell Line (HEp-2) By Different Concentrations Of Quercetin Was Done To Assess The Viability Of The Treated Cells To Determine The Percentages Of Viable Cells That Was Monitored Using MTT Assay.
Results: The Mean Viability Percentage Of Quercetin Treated Cells (in Relation To Control Cells) Increased As Quercetin Concentration Decreased From 640 MM To 5mM With An IC50 Of 200 MM. Regarding The Cytotoxic Effect Of Quercetin And Related Cell Cycle Profile It Was Noticed That Cell Distribution Showed A Variable Percent Of Arrest At Different Phase Of Cell Division Profile, Where There Was Non Significant Difference Of Arrested Cells Of Quercetin Pre And Post Treatment Compared With Its Value In Non Treated G0-G1 Phase Control Cells(P>0.05) And S Phase As Well. While There Was A Significant Elevated Arrest Of Treated Cells During The G2-M Phase (P<0.05) And The Significant Difference Of Cell Arrest At G2-M Phase Was Type Of Treatment Related .
Conclusion: From The Results Of The Current Study, We Found That Quercetin Has Cytotoxic Effect On Squamous Cell Carcinoma, (HEp-2) Cell Line, It Also Induced An Effect On The Cell Cycle Distribution, And Quercetin Has Effect On (HEp-2) Cell Line Resulting In Apoptosis And Necrosis.
RECENT ARTICLES
Most Viewed