Mohammed Rana, Negm.Maged, Shaker Olfat And El-khodary. Sherif,

ABSTRACT
Aim : To Evaluate The Effect Of TotalFill Bioceramic Sealer Versus AH Plus Resin Sealer On Cell Viability And To Compare The Effect Of The TotalFill Bioceramic Sealer Versus AH Plus Resin Sealer On Diameter Of Zones Of Bacterial Inhibition On Enterococcus Faecalis, Porphyromonas Gingivalis And Porphyromonas Endodontalis. Methods: Cytotoxicity Was Analyzed On Baby Hamster Kidney�fibroblasts� (�BHK) Submitted To Cell Culture Medium Conditioned By Sealers Using The MTT Reduction Assay. Cells Grown On Fresh Medium Served As Control. Cell Viabilities Were Measured At 24, 48 And 72 Hours. The Percentage Of Viable Cells In Each Well Was Calculated. The Antibacterial Activity Was Analyzed On An Enterococcus Faecalis Strain (ATCC 29212), Porphyromonas Gingivalis And Porphyromonas Endodontalis Using Agar Diffusion Test(ADT). The Inhibition Zones In ADT Were Measured After 48 Hours.

Results: Cultures Submitted To TotalFill BC Sealer Had A Significantly Higher Number Of Viable Cells (p<0.05) Than AH Plus Sealer. TotalFill BC Sealer Exhibited Significantly Smaller Inhibition Zones Than AH Plus With (p<0.05) On Enterococcus Faecalis, While It Showed Statistically Significant Larger Inhibition On P.endodontalis And On P.gingivalis Than AH Plus . Conclusion: The Bioceramic-based Sealer (TotalFill) Has Higher Biocompatibility Than The Resin Sealer (AH Plus). TotalFill Sealer Has Lower Antimicrobial Effect On E.faecalis And Higher Antimicrobial Effect On P.endodontalis And P.gingivalis In Comparison To AH Plus Sealer.