Merna Ageeb Kamal , Sherif Farouk El Gayar And Hanan Eid Gamal,

ABSTRACT
Background: Grape Seed Extract (GSE) Has An Effective Anti-cancer Property Against Squamous Cell Carcinoma. The Aim Of The Study Is To Identify The Effect Of GSE On Squamous Cell Carcinoma Cell Line (HEP-2). Material And Method: Treatment Of Cell Line (HEp-2) By Different Concentrations Of GSE Was Done To Assess The Viability Of The Treated Cells To Determine The Percentages Of Viable Cells That Was Monitored Using MTT Assay. Using Annexin V And Caspase III For Determination Of Apoptosis.

Results: The Mean Viability Percentage Of GSE Treated Cells (in Relation To Control Cells) Decreases From 86.67 To 46.32 As GSE Concentration Increased From 0.39 ?g To 100 ?g. Morphometric Analysis Showed A Decrease In Nuclear Area Factor (NAF) Which Is An Indicator Of Apoptosis As Concentrations Increased. The Results Were Statistically Significant. Cell Cycle Analysis Showed Pre-G1 Apoptosis Produced By The Different Concentrations Of GSE. The Percentages Of Cells In Pre-G1 And G2/M Phases Increased With Control Cells. The Detection Of Caspase III Showed That Higher Level Of Caspase 3 Was Detected In Post IC50 Concentration Group, While The Lower Level Was Detected In The Control Group. Conclusion: GSE Has A Significant Anticancer Effect On Hep-2 Cell Line.