Laila Hussein Ibrahim El-Mansy, Magdy Mohamed Ali , Reham EL Sayed Hassan Khaled Ali Beshr And Salma Hassan El Ashry,

ABSTRACT
This Research Work Was Executed To Assess And Relate The Cyto-toxic Outcome Of A Recently Introduced Tricalcium Silicate Bioceramic Sealer (BioRoot? RCS) In Parallel With A Salicylate-resin Based Sealer (MTA-Fillapex). Sealer Discs With 5mm In Diameter And 2mm In Height Were Prepared Using Teflon Molds. The Effects Of Sealer Extracts On Cell Vitality And Proliferation Were Evaluated Using A Methyl-thiazoldiphenyl-tetrazolium (MTT) Assay. The Experimentations Were Executed Using MRC-5 Cells At 37 ?C In 100% Humidified Atmosphere Containing 5% CO2. Cells Were Subjected To Different Concentrations Of The Tested Sealers? Extracts And Cytotoxicity Was Assessed At 24 Hrs. And 72 Hrs. Relative To Controls. Data Were Explored For Normality Using Kolmogorov-Smirnov Test. For Parametric Data, Independent T Test Was Used To Compare All Groups. Paired-t Test Was Utilized For Comparing The Same Group At Different Observation Times. The Significance Level Was Set At P ? 0.05. BioRoot-RCS Was Significantly Less Cytotoxic Than MTA-Fillapex With A Proliferative Time Dependent Profile.