Doaa Abdel Aziz Elbedewy*, Wedad Mohammed Etman, Thuraia Mohammed Genaid And Olfat Gaballah,

ABSTRACT
Objective: The Purpose Of This Experimental Study Was To Treat Induced Demineralized Coronal Dentin Using Biomimetic Material.

Materials and Methods: A Total Of 6 Healthy Dogs Were Used In This Study. The 2nd & 3rd Upper And Lower Incisors Were Used Representing The Tested Groups. The Labial Enamel Was Removed, And Exposed Dentin Surfaces Of All Experimental Specimens Were Acid Etched. Specimens In Group I Were Treated By Agarose Hydrogel Mineralization System, While In Group II They Were Subjected Only To Oral Saliva. The Hydrogels Were Applied To The Dentin Surfaces For 5 Hours. This Procedure Was Repeated Twice Weekly With 3 Days Intervals. Two Dogs Were Used For Each Of The Three Test Periods Of Material Application (15, 30 And 45 Hours). Before Scarifying The Dogs, The Lower Left 2nd & 3rd Incisors Were Extracted Representing Control Specimens. The Morphology And The Chemical Composition Of All Dentin Surfaces Were Investigated By Using Scanning Electron Microscope Coupled With Energy Dispersive X-ray Spectrometry, And All Data Were Collected And Subjected To Statistical Analysis Using SPSS Version 20. Comparisons Between The Tested Groups And The Controls Were Conducted Using One- Way ANOVA.

Results: SEM Images Of Sound Dentin Revealed The Smear Layer Was Covering And Occluding Some Of The Dentinal Tubules In The Control Group. The Images Of Group I After 15 Hours Of Treatment Revealed The Deposition Of Some Minerals On The Walls Of The Opened Dentinal Tubules Occluding Some Of Them, And EDAX Analysis Presented A Significantincrease In (Ca) Value From (23.08) To (25.92). These Images Still Seen After 30 Hours, While After 45 Hours The Images Showed That Almost The Tubules Were Partially Obliterated With Enamel Prism-like Structures. In Group II, Images Showed Opened & Partially Occluded Dentinal Tubules, And The EDAX Analysis Showed Significantincrease In (P) Value From (7.91) To (11.58). The Previous Findingalso Found After 30 Hours And Subjecting The Teeth For 45 Hours To Oral Saliva The Images Showed Fully Opened Dentinal Tubules With A Significant Increase In (Ca) Value From (6.90) To (14.51).

Conclusions: Group I Resulted In Partially Obliterated Dentinal Tubules With Enamel Prism-like Structures, While The Oral Saliva In Group II Was Not Effective Enough To Treat Dentin Erosion Currently.