Fatma Abd El Nasser Abd El Rehim Abouel Maaty, Samah Sayed Mehanni And Zeinab Amin Salem,

ABSTRACT
Background: Tongue Cancer Is A Serious Life Threatening Type Of Oral Cancer. Egyptian Propolis Has Many Biological Activities As Cancer Inhibitory Activity Owed To Its Content Of Phenolic Compounds And Esters.Objective: The Present Study Aims To Monitor The Anticancer Effect Of The Egyptian Propolis Methanolic Extract Through Ultrastructural Study, COMET Assay, MTT Assay, MiRNA Expression And Morphological Changes By Inverted Microscopy.

Materials and Methods: Tongue Carcinoma Cells Grow Without Any Intervention As Group I. Propolis Extract Was Prepared From Crude Egyptian Propolis And Applied On Human Tongue Cancer Cells As Group II. Phytochemical Analysis Was Made For The Propolis Extract. Anticancer Efficacyof Group II Was Investigated By The Mentioned Techniques.

Results: Significantcytotoxic Effect, Ultrastructural And Morphological Apoptotic Changes In Cancer Cells Were Detected In Group II. At 24 And 48 Hrs Time Interval MTT Assay Showed A Significantdrop In Cell Viability From Group I To Group II, COMET Assay Showed An Increase In % Of Apoptosis From Group I To Group II And Both MiRNA 21 And MiRNA 195 Were Dropped Significantlyin Group II.Conclusion: This Study Shows That Egyptian Propolis Methanolic Extract Proved To Have Apoptotic Effect On Tongue Carcinoma Cells.