Eman Ahmed Abu Bakr , Nihal Ezzat Sabet And Shaimaa Ismail Gawdat,

ABSTRACT


Introduction: The Purpose Of This Study Was To Assess The Antibacterial Activity Of BioRoot?RCS Sealer And AH Plus Sealer In Comparison To Tubli SealTM Sealer On Enterococcus Fecalis Biofilmby Using Dentin Block Infection Model And Confocal Laser Scanning Electron Microscope. Methods: E. Faecalis Bacteria Was Used As A Test Organism. Forty Two Dentin Specimens Were Prepared From A Human Single Rooted Teeth Infected With E. Faecalis Biofilmsand Incubated For 3 Weeks. The Tested Sealers (BioRoot? RCS Sealer, AH Plus Sealer And Tubli SealTM Sealer) Were Mixed According To Manufacturer?s Instructions, Then Added To The Root Canal Wall Of The Dentin Specimen In Equal Thickness And Placed At 370C In 100% Relative Humidity For 1, 7, And 30 Days. The Ratio Of Dead And Live Bacteria Inside The Dentinal Tubule Was Measured By Confocal Laser Scanning Microscope.

Results: More Bacteria Were Killed In The Two Sealer Groups (BioRoot? RCS Sealer, AH Plus Sealer) Than Tubli SealTM Sealer. BiorootTM RCS Sealer And Tubli SealTM Sealer Showed A Statistically Significantbacterial Killing At All 3 Time Points (p<0.05). AH Plus And Tubli SealTM Sealers At 7 Days Showed No Statistical Difference But At 30 Days, The 3 Sealers Were Statistically Different.

Conclusions: The 3 Endodontic Root Canal Sealers Had Antibacterial Effects Against E. Faecalis In The Dentinal Tubules. Bioroot? RCS Sealer And AH Plus Had Superior Antibacterial Effects Compared To Tubli SealTM Sealer.