ANTIMICROBIAL EFFECTS OF AQUEOUS EXTRACTS OF CINNAMON AND GINGER IN COMPARISON TO CHLORHEXIDINE GLUCONATE 0.2% ON THE ORAL STREPTOCOCCUS MUTANS (IN VIVO STUDY)

Nadhel A. Mohamed , Heba Salah El-Dein Hamza And Mai Mamdouh El-Sayed,

ABSTRACT


Aim: To Assess The Antimicrobial Effect Of Aqueous Extract Of Cinnamon 20% And Ginger 20% In Comparison Chlorhexidine 0.2% On Oral Streptococcus Mutans.

Materials and Methods: Twenty One Participants Selected For The Study, Were Divided Into Three Groups (N=7), Where Group (R1); Cinnamon 20%, Group (R2), Ginger 20% And Group (R3), Chlorhexidine Gluconate 0.2 % Mouth Rinse. Each Volunteer Was Given A Piece Of Arabic Gum And Asked To Chew It For One Minute Only, Then Stimulated Saliva Was Collected In Sterilized Screw Capped Bottles. After One Minute, Each Volunteer Was Asked To Rinse With Ten Ml Of The Tested Agent For One Minute Then Expectorate. Stimulated Saliva Was Recollected In The Following Points: After 30 Minute, 1 Hour And 2 Hours. Salivary Samples Were Homogenized By Vortex. 0.1 Ml Of Saliva Transferred To 0.9 Ml Of Sterile Phosphate Buffer Saline (pH 7.0), And Then Ten-fold Dilution Was Performed. From Dilution 10-3 Of Salivary Samples 0.1 Was Taken And Spread In Duplicate On The Mitis Salivarius Bacitracin Agar Media. The Plates Were Incubated Anaerobically For 48 Hours At 37�C Then Aerobically For 24 Hours At Room Temperature. The Number Of Colonies Was Expressed As Colony�forming Units Multiplied By The Dilution Factor Per Milliliter Of Saliva (CFU/ml) And Compared Before And After Rinsing.

Results: Chlorhexidine Demonstrated The Highest Efficacy Than The Other The Two Herbal Plants In Bacterial Reductions But This Was Only Statistically Significant When Comparing The Effect Of Chlorhexidine 0.2% To Ginger 20% Cinnamon 20% Performed Better Than Ginger 20% But The Mean Difference Was Not Statistically Significant. Conclusion: Although The Herbal Extracts Were Less Potent Than Chlorhexidine, Still They Were Relatively Effective In Inhibiting The Growth Of Oral Bacteria. Cinnamon 20% Has More Antibacterial Activity On The Growth Of Streptococcus Mutans Than Ginger 20%.

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