Abd El-Ghany L.B., El-Hossary W.H. And Hassan M.M.A,
ABSTRACT
Objective: The Aim Of This Study Was To Evaluate The Expression Of P53 Gene Following Treatment Of Induced Oral Cancer, In Hamster Buccal Pouch (HBP), With 0.001 Mg/kg Body Wt Thymoquinone (TQ) Loaded On Gold Nanoparticles (GNPs).
Materials and Methods: The Study Was Done On Archival Paraffin Blocks To Study The Effect Of Different TQ Preparations On The Chemically-induced Squamous Cell Carcinoma (SCC) In The (HBP). The Chemical Carcinogen 0.5% 7,12 Dimethyl Benz-(a)-anthracene (DMBA), Was Topically Applied To HBP For 14 Weeks. The Paraffin Blocks Represented Buccal Pouches From 55 Male Syrian Golden Hamsters, Weighed 90-120 Grams. They Were Divided Into 4 Major Groups: Group A (control Groups): A1 (20 Animals) Served As Negative Control Group (did Not Receive Any Treatment), And A2 (5 Animals) Served As Positive Control (painted With DMBA For 3 Times/week For 14 Weeks). Group B: (30 Animals) As The Experimental Groups (painted With DMBA 3 Times/week For 14 Weeks) And Then Divided Into Group B1:5 Animals Were Euthanized After 3 Weeks Of I.p Injection Of 0.001 Mg/kg Body Wt TQ, Group B2: 5 Animals Were Euthanized After Six Weeks Of I.p Injection Of TQ, Group B3: 5 Animals Were Euthanized After 3 Weeks Of I.p Injection Of GNPs, Group B4: 5 Animals Were Euthanized After 6 Weeks Of I.p Injection Of GNPs, Group B5: 5 Animals Were Euthanized After 3 Weeks Of I.p Injection Of TQ-GNPs And Group B6: 5 Animals Were Euthanized After 6 Weeks Of I.p Injection Of TQ-GNPs. H&E Stain Was Performed To Confirm The Histopathological Changes And Immunohistochemical Stain For P53.
Results: Positive Control And Experimental Groups (B1-B5) Showed Different Degrees Of Epithelial Dysplasia And Intense P53 Expression(+3) Whereas Group B6 Showed Moderate P53 Immune Reactivity (+2) With Focal Areas Of Variable Dysplastic Grades Along The Epithelial Lining. I.e, The Most Effective Treatment Was Obtained Only Through This Group.
Conclusion: TQ (0.001 Mg/Kg Body Wt) Loaded On GNPs, Was Effective In Partial Restoration Of P53 Activity, Especially When Used For 6 Weeks. The Follow Up-timing Of The Present Study Appears Short To Explore The Full Effect Of TQ-GNPs On That Model Of Induced-SCC.
