Ahmed G. Ahmed , Dalia M. Fayyad And Nihal E. Sabet,

ABSTRACT
This Study Aimed To Evaluate The Antimicrobial Effect Of Salvadora Persica (Miswak), Sterilox (super-oxidized Water) And Peractic Acid In Comparison With Sodium Hypochlorite When Used As An Irrigant In Infected Root Canals On 3 Types Of Bacteria Staphylococcus Aureus, Escherichia Coli And Enterococcus Faecalis.

Materials and Methods: One Hundred Recently Extracted Human First Maxillary Molars Were Collected With Their Palatal Roots Cut From The Coronal Portion To A Standardized Length Of 12mm. Then Root Canals Were Infected With Bacteria And Incubated For One Week. Root Canals Were Classified Into Four Groups (n=20) According To The Type Of Final Irrigant Used As Follow: Group I Was Treated With Salvadora Persica 50% ,Group II Was Treated With Sterilox, Group III Was Treated With Peractic Acid 2.5%, Group IV: Was Treated With Sodium Hypochlorite 2.5%. Two Other Groups (n=10) Were Positive And Negative Control. A Classical Bacterial Count Technique With The Aid Of Colony Counter Machine Was Used On The Agar Plates, So The Mean Value Of Colony Forming Units For The Plates Of Each Group Was Calculated By The Quantitative Method As A Measure Of The Number Of Viable Bacteria Existed. To Determine The Penetration Of Viable Bacteria Into The Dentinal Tubules, Dentin Shavings Was Obtained From The Samples By Using #3 And #5 Gates Glidden Drill. Debris Collected On The Flutes Of Each Drill Was Placed In Eppendorf Tube Containing Saline And Vortexed For 30 Seconds. Then The Infected Saline Was Placed On Nutrient Agar Plates And Incubated For 48 Hours.

Results: Regarding The Antibacterial Effect In The Three Time Intervals (immediate, 48 Hours And 3 Weeks) Showed That NaOCl Was The Most Effective Irrigant Among All Irrigants Used, Followed By Peracetic Acid, Sterilox And Then Followed By Salvadora Persica. Regarding The Penetration Showed That Peracetic Acid Was The Most Effective Followed By NaOCl, Sterilox And Then Followed By Salvadora Persica. Conclusion: Sodium Hypochlorite Was The Best Antibacterial Irrigant Used, Yet Peracetic Acid Was Very Promising As It Had Antibacterial Effect Close To Sodium Hypochlorite But It Acts Also As A Chelating Agent.