Gehan Abdel Naser Abdel Rahman, Samia Mostafa El-Azab, Amr Helmy El Bolok , Sherif Farouk El-Gayar And Aly Fahmy Mohamed,

ABSTRACT
Background: Sinapic Acid Is One Of The Phenolic Acids That Widely Distributed In Edible Plants Such As Cereals, Nuts, Oil Seeds And Berries. Sinapic Acid Shows Antioxidant, Antimicrobial, Anti-inflammatory, Anticancer, And Anxiolytic Activity.

Materials and Methods: The Present Study Was Conducted Based On Monitoring Of In Vitro Cytotoxicity Using MTT Assay, Morphological Changes Determination Of Free And Nano-capsulated Sinapic Acid Using Histopathological Examination And Image Morphometric Analysis. Nano-capsulation Was Performed Using Tween-80, And Biochemical Changes Concerning Reactive Oxygen Species (ROS) Profile.

Results: Data Recorded Revealed That The Inhibitory Concentration (IC50) Of Free And Nano-capsulated Sinapic Acid Was 646.4 ?M/ml And 84.74 �M/ Ml Post Treatment Respectively. Also, Cytotoxicity Was Concentration Dependent With Significant Difference Between Free And Nano-capsulated One (P<0.05). Also, ROS As A Biomarker Was Elevated In A Significant Way Than In Case Of Nano-capsulated Sinapic Acid. Cytotoxicity Accompanied With Morphological Changes Denoting Apoptosis, Necrosis And Secondary Necrosis After Treatment With Free And Nano-capsulated Sinapic Acid. In The Meantime The Biochemical Marker (ROS) Was Changed Post Cell Treatment With Both Formulae Of Sinapic Acid. Conclusion: Nano-capsulated Sinapic Acid Is More Effective As Cancer Chemopreventive Agent With Much Lower Concentration Than Free Sinapic Acid On Squamous Cell Carcinoma Cell Line.