Rania Khedr Ahmed, Amany Mohammed Korsel And Waleed Elshahawy,

ABSTRACT


Purpose: The Aim Is To Evaluate The Effect Of Zirconia And Porcelain Fused To Metal Crowns On DNA Of Dog?s Gingival Fibroblast (casepase Gene) Using Polymeraise Chain Reaction (PCR) Test.

Materials and Methods: Fifteen Adult Dogs Were Selected. Five Of Them Were Used As Control. Lower Left Canines Of Remaining Dogs Were Prepared To Receive Porcelain Fused To Metal (PFM) And Zirconia Crown. After Three Months From Insertion Of The Crowns, Samples Of Gingiva Were Taken From All The Dogs And Polymerase Reaction (PCR) Test Was Performed On Casepase Gene And The Process Was Repeated After Six Months. The Data Collected From PCR Were Statistically Analyzed Using Independent-samples T Test.

Results: After Three Month, The Mean Of Casepase Gene In Dogs Had Porcelain Fused To Metal Crowns PFM Was (0.92�0.54) And In Dogs Had Zirconia Crowns Is (0.62�0.41) And After Six Months, The Mean Of Casepase Gene In The Dogs Had PFM Crowns Was (0.93�0.42) And In The Dogs Had Zirconia Crowns Is (0.66�0.48). There Is Positive Correlation Between Three And Six Months In Porcelain Fused To Metal (r=0.98). There Was Also Positive Correlation Between Three And Six Months In Zirconia(r=0.99). Casepase Gene Decrease In Porcelain Fused To Metal By 7.2% And In Zirconia By33.8% After Six Months. Conclusion: Within The Limitations Of This Study, Casepase Gene Decrease In Case Of Zirconia More Than PFM Restoration. The Reduction In The Rate Of Gene Means That Apoptosis Occurred In Both Material But It Occurred In PFM More Than Zirconia Restorations So Zirconia Is More Biologically Accepted Than PFM.