ASSESSMENT OF ANTIMICROBIAL EFFECT AND SHEAR BOND STRENGTH OF SILVER NANO PARTICLES MODIFIED RESIN CEMENT

Manal R. Abu-Eittah, Raiesa M M Hashem And Asmaa A Mohammed,

ABSTRACT
The Aim Of This Study Was To Investigate The Antimicrobial Effect And Shear Bond Strength Of Silver Nanoparticles Modified Resin Cement.

Materials and Methods: A Total Of 40 Samples Were Constructed And Classified Into Two Groups (20 Samples For Each). Group I (control Group) Samples Without Any Modification And Group II (Resin Cement Samples Modified With Silver Nanoparticles 30-80nm (Nanotech-Egypt). Each Group Was Tested For Antimicrobial Activities(15 Samples For Each Shaped As Composite Resin Cement Discs Of 2mm Thickness And 5mm Diameter), Samples Were Tested Against Three Types Of The Microorganisms, (Escherichia Coli (gram ?ve Bacteria ), Staphylococcus Aureus (gram +ve Bacteria) And Candida Albicans (yeast) 5 Samples For Each . Colonies Forming Unit (CFU) Counts Were Used To Test The Antibacterial And Antifungal Effect Of Silver Nanoparticles. For The Shear Bond Strength Test 10 Upper Molars Collected And The Occlusal Enamel Was Removed Then 10 Feldspathic Porcelain Discs Fabricated And Cemented On The Occlusal Surface (5 Samples For Each) Results Were Tabulated And Statistically Analyzed Using One Way ANOVA Test For Quantitative Data Followed By Post Hoc Tukey Analysis Between Each Groups And The Significance Level Was Set At P � 0.05. Result: Modified Group Exhibited Antimicrobial Effect Toward Gram-negative (E. Coli), Gram-positive (S. Aureus) As Well As To The Yeast (C. Albicans). Where The Microbial Percentage Inhibition (Escherichia Coli 45.4�3.17, Staphylococcus Aureus 33.2�2.12 And Candida Albicans 34.2�1.64). The Effects Of The Tested Group Were More Pronounced On E-coli Than Staphylococcus Aureus. There Was Non-significant Difference In The Shear Bond Strength Between The Control Group And The Modified Group. Conclusion: The Addition Of Silver Nanoparticles To Resin Cement Was Proved To Be A Very Effective Method In Inhibition Of Bacterial Growth Via Decreasing The Colonizing Activity. The Antibacterial Effects Of Both Tested Materials Were More Pronounced On Gram ?ve Than Gram +ve Bacteria. There Was Non-significant Decrease In Shear Bond Strength Between The Control And The Modified Group. Key Words: Silver Nanoparticles, Anti-bacterial Materials, Shear Bond Strength, Resin Cement.

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