Doha Mohammed Afifi, Amal Hassan Abdel-Rahman And Heba Mahmoud Dahmoush,
ABSTRACT
Background: Oral Squamous Cell Carcinoma Is A Malignant Tumor With Poor Prognosis And Therefore The Discovery Of Markers For Early Signs Of Malignant Transformation Would Be Of Critical Importance In Clinical Diagnosis. In This Study We Aimed To Detect The Changes In ANXA1 Immunoexpression In Tissues Of Normal Rat Tongue Mucosa And In Induced Dysplasia And OSCC.
Materials and Methods: 25 Rats Were Used, Divided Into 2 Groups: A Control Group (5 Rats With No Induction) And Experimental Group (20 Rats Which Received Topical Painting Of DMBA And Formaldehyde For Induction Of Dysplasia And SCC). Tongue Dissection Was Performed At Euthanization Dates. Area Percent Of Immunohistochemical Expression Of ANXA1 Was Measured By Software Leica Qwin 500.
Results: ANXA1 Was Expressed In The Cell Membrane Of Normal Tongue Mucosa And Loss Of This Expression Was Frequent In Both Dysplasia And OSCC. A Statistically Significant Difference In ANXA1 Expression Was Present Between Normal Tongue Mucosa And Dysplastic Lesions Or OSCC.
Conclusion: ANXA1 Immunostaining Has A Potential Utility For The Detection Of Epithelial Dysplasia.